Aspect and Input Issues happens only in Build version

Hi, there:

I'm making a 2D vertical display game. When testing in game mode, everything work properly. However, when trying to build a PC or Mac standalone version, the outcome shows only part of the whole image, with many game object lost, and the keyboard input no longer work at all.
I've changed those display setting such as default screen width and height in player setting such, and also add the keys in the input manager, but none of them work.

Any suggestion will be quiet appreciated!

adenovirux